Composite
Part:BBa_K5335023:Design
Designed by: Ruichen Shen Group: iGEM24_HZAU-China (2024-09-28)
A part (including a reporter gene) that induces and regulates downstream gene expression by salicyli
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 786
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 77
Illegal NgoMIV site found at 618
Illegal AgeI site found at 1855 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Note that activation of Psal by NahR is dependent on the NahR binding sequence, which may not be annotated in some of the original documents.
Source
NahR, NahR binding sequence, and Psal from the naphthalene operon of Pseudomonas putida.